Retinal OCT layers are distinct strata within the retina discernible by optical coherence tomography (OCT). These layers correspond to specific anatomical structures with unique functions. The nerve fiber layer (NFL) represents the axons of retinal ganglion cells, while the ganglion cell layer (GCL) contains their cell bodies. The inner plexiform layer (IPL) and inner nuclear layer (INL) are synaptic layers, where neuronal connections are formed. The outer plexiform layer (OPL) and outer nuclear layer (ONL) are involved in photoreceptor signaling. Understanding the OCT layers enables clinicians to precisely locate and assess retinal abnormalities, aiding in the diagnosis and monitoring of various ophthalmic conditions.